Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K2150024:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pCon+RBS+tetR+DT+pTet+RBS+GFP+DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1615


Design Notes

This part is used for logic verification. Whether green fluorescence is observed serves as an indicator whether the system works. In real use, substitute GFP for target gene.


Source

pCon from BBa_J23119, RBS from BBa_B0034, tetR from BBa_C0040, DT from BBa_B0015,PTet from BBa_R0040, GFP from BBa_E0040.

References